MRI Sequence Development

The MRI sequence development team focuses on the development and optimization of new magnetic resonance imaging (MRI) sequences and protocols tailored to several preclinical applications for Bruker and Aspect Imaging MRI scanners. Led by Dr. Walter Dastr ú and Dr. Dario Longo, the team specializes in MRI data acquisition, image reconstruction and data analysis, covering gadolinium-based, 19F and CEST-MRI applications for improving acquisition time, volume coverage and contrast. Developed sequences are free for downloading by all interested research parties. If the sequences are used for publication purposes, acknowledgments in the manuscript about the sequence are appreciated.

• A single-shot single-slice sequence for CW-CEST imaging
A single-shot centric encoding RARE-based sequence with an implemented frequency offsets loop that allows to store all the images sampled with different frequencies (with CW saturation) in a single MR scan. This package contains the files and the instructions for a multislice CEST sequence installation and first use. The sequence is based on a RARE method developed for Bruker MRI scanners running with ParaVision 5.1 and with a modified Magnetization Transfer module.
More information about the sequence is available in:
"Iopamidol as a responsive MRI-chemical exchange saturation transfer contrast agent for pH mapping of kidneys: In vivo studies in mice at 7 T", Longo DL et al;
Magn Reson Med, 2011, 65:202-11, doi: 10.1002/mrm.22608
Download link for ParaVision 6.0.1
Contacts: dario.longo@unito.it

• A RARE-based sequence for fast multislice CEST imaging
A fast multislice CEST acquisition scheme has been implemented by splitting the saturation module into two parts: a first long saturation pulse that generates the steady-state and a second short saturation pulse, repeated before each slice, to restore saturation losses. Each slice is acquired with a centric-encoding single-shot fast spin echo (RARE) readout. This package contains the files and the instructions for a multislice CEST sequence installation and first use. The sequence is based on a RARE method developed for Bruker MRI scanners running with ParaVision 6.0.1/360.1.1.
More information about the sequence is available in:
"A fast multislice sequence for 3DMRI-CEST pH imaging", Villano D et al;
Magn Reson Med, 2021, 85, 1335-1349. doi:10.1002/mrm.28516
Download link for ParaVision 6.0.1
Download link for ParaVision 360.1.1
Download link for ParaVision 360.2
Contacts: dario.longo@unito.it

If you find our CEST sequence useful, please consider citing:
D. Villano et al; "A fast multislice sequence for 3DMRI-CEST pH imaging", Magn Reson Med, 2021, 85, 1335-1349.
doi:10.1002/mrm.28516

Research groups using our sequences:

The sequences are provided for free but to download them please fill out the form. Of course, their use is under the operator's responsibility.
All information collected will be stored securely and will never be used for commercial purposes.



Group Leaders:

Dario Longo Walter Dastrù